I'm not so sure.... the inputs to the ECU do not include TBS flow. It certainly wasn't true on Gen1/II. But again, all you want to do is achieve the perfect balance if that is possible with the one screw in all the way (no flow). I have no problem experimenting a bit, the only hesitation is if it affects idle speed, which is not adjustable on the GenIII. If it does have a negative effect, it's all reversible, no harm done and we'll learn something about the unknown.
My thought is with 1 cylinder having no flow and the others some, what effect that has..... of course, as roaddog has mentioned, there are other factors that affect vibration and some are beyond our control (we don't have a blueprinted engine, plugs can't be perfect all the time, perfect combustion, etc.)
, if TBS is adjusted at idle, then run up to 4000, at least on GenII tweaking it so the 4000 rpm balance was perfect had a significant effect on idle TBS..... traditional thinking was it was better to have the best balance at idle..... who knows, I can't recall anyone tweaking the 4000 balance and running with it.
Perhaps worth a shot, again all reversible if it doesn't pan out.
I have done this on my GenI 2005 model, balanced them at 4K and I had good results. Idle balance was close enough, a lot closer than the same attempt I recent did on my GenIII.
From all I've read the GenIII balance is to be made at idle and using cyl 1 as the reference so I readjusted, without test riding, back to a balanced idle.
That is the reasoning for my questioning. (If the Gen1 was to be done the same way, well I missed that instruction.)
After the re-balance on my GenIII my idle seemed better with the clutch pulled in where before it was pretty inconsistent. As far as smoothness at higher rpm I couldn't tell much difference, mine is really very smooth with what I call a rough spot around 4k that most people speak of, my 05 had the same but more pronounced but was tamed with the 4k balancing act.
Now, at the time I did the 05 TBS I also changed the plugs,(valve adj. was 10K before this adjustment) did that make the difference I noticed or the TBS? Or maybe the fuel I used in it? My 05 was kinda particular on what fuel I fed it. My 2013 doesn't seem to be as fuel- finicky IMO.
BTW, air temp also makes a difference, anyone else noticed how well your bike runs with cool dense air?
So after reading the comments from others that understand the mechanics of hows things work I come to the conclusion that there are many things involved besides a simple air screw adjustment.
This inline 4cyl engine design is a pretty smooth motor, if the engineers wanted to take all vibrations out of it maybe they should have rubber mounted it?
If some can stand it, try a V4 or an opposed 6cyl.